Enzyme KineticsIn an enzyme reaction S → P, the rate of the reaction is depend on the substrate concentration. At low substrate concentration the rate of reaction ‘v’ is proportional to substrate concentration. As the substrate concentration is incr…
Continue ReadingMECHANISM OF ENZYME ACTIONTwo hypotheses have been put forward to explain the mode of enzyme action.01.Lock and key hypothesis: This hypothesis was given by Email Fischer in 1984. According to this hypothesis, both enzyme and substrate molecules hav…
Continue ReadingNOMENCLATURE AND CLASSIFICATION OF ENZYMESEnzymes are generally named by adding “ase’ to the root indicating the substrate on which the enzyme acts. The International Union of Biochemistry report of 1962 contain a scheme for enzyme classification. E…
Continue ReadingHow do Enzymes Catalyze ReactionEach enzyme has an active site to which substrate binds and forms a short-lived highly reaction enzyme substrate complex. This is followed by enzyme product complex (EP). Finally the enzyme product complex dissociates…
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